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NAD+ 500 mg vial: mixing and syringe units

A 500 mg vial of NAD+ mixed with 5 mL of bacteriostatic water is 100 mg/mL. At that strength 25 mg is 25 units on a U-100 syringe and 100 mg is 100 units. The doses on this page are a reported range, not a label dose.

Last checked 10 Oct 2026

500 mg vial

Water 5 mL

Concentration 100 mg/mL

US Not approved

Canada Not authorized

Checked 10 Oct 2026

NAD+ 500 mg draw chart

Each row is one dose, the volume to draw and where that sits on a U-100 insulin syringe. The last three columns show which syringe sizes can hold it in one draw.

NAD+ 500 mg vial with 5 mL of bacteriostatic water, 100 mg/mL. 1 to 3 times weekly.
DoseVolumeSyringe units0.3 mL syringe0.5 mL syringe1 mL syringe
25 mg0.25 mL25 unitsYesYesYes
50 mg0.50 mL50 unitsNoYesYes
100 mg1.00 mL100 unitsNoNoYes

Source Reported step-up, not a label dose: 25 to 50 mg in weeks 1 and 2, then 50 to 100 mg. Published human studies gave NAD+ into a vein. Vial math is arithmetic.

Open it in the calculator

How to mix a 500 mg NAD+ vial

  1. Wash your hands. Wipe the stopper of the NAD+ vial and the water vial with an alcohol swab.
  2. Draw 5 mL of bacteriostatic water into a syringe.
  3. Push the needle through the stopper of the peptide vial and let the water run slowly down the glass, not straight onto the powder.
  4. Swirl the vial gently until the powder is gone. Do not shake it.
  5. Write the date and 100 mg/mL on the vial, then put it in the fridge.

If you add a different amount of water

More water makes each dose a bigger, easier draw. Less water makes it smaller. The milligrams in the vial do not change.

Water addedConcentration25 mg50 mg100 mg
1 mL500 mg/mL5 units10 units20 units
2 mL250 mg/mL10 units20 units40 units
3 mL166.667 mg/mL15 units30 units60 units
5 mL100 mg/mL25 units50 units100 units

The marked row is the amount used in the chart above. Red cells are draws under 5 units, which are hard to read, or over 100 units, which one 1 mL syringe cannot hold.

How many doses are in the vial

DoseWhole doses in a 500 mg vial
25 mg20 doses
50 mg10 doses
100 mg5 doses

How long the vial lasts depends on how often it is used. The reported frequency for NAD+ is: 1 to 3 times weekly.

NAD+ 500 mg vial questions

How many units is 25 mg of NAD+ from a 500 mg vial?

With 5 mL of bacteriostatic water the vial is 100 mg/mL. 25 mg is 0.25 mL, which is 25 units on a U-100 syringe.

How many units is 50 mg of NAD+ from a 500 mg vial?

With 5 mL of bacteriostatic water the vial is 100 mg/mL. 50 mg is 0.50 mL, which is 50 units on a U-100 syringe.

How many units is 100 mg of NAD+ from a 500 mg vial?

With 5 mL of bacteriostatic water the vial is 100 mg/mL. 100 mg is 1.00 mL, which is 100 units on a U-100 syringe.

How much bacteriostatic water goes in a 500 mg NAD+ vial?

5 mL is the amount used on this page. It gives 100 mg/mL and puts the doses at 25, 50, 100 units. Any amount works as long as the math is redone for it.

What if I added 1 mL instead of 5 mL?

The vial would be 500 mg/mL instead of 100 mg/mL, so 25 mg would be 5 units instead of 25. The dose in the syringe is the same; only the volume changes.

How many doses are in a 500 mg NAD+ vial?

Whole doses in one vial: 20 at 25 mg, 10 at 50 mg, 5 at 100 mg.

More on NAD+

This page is only the math for one vial size. Where the doses come from, what the studies found, the side effects and the sources are on the main page.

NAD+ dosageWhat NAD+ isHow to mix a vialHow to read a syringe

DosageFacts is an information site. It is not medical advice. Talk to a licensed professional before using any medication.