Cardiogen dosage: chart, reported range and vial math

How to mix a Cardiogen vial

These steps are for the 20 mg vial picked in the chart. Change the size there and the amounts here change with it.

1. Wash your hands. Wipe the stopper of the peptide vial and the water vial with an alcohol swab.
2. Draw 2 mL of bacteriostatic water into a syringe.
3. Push the needle through the stopper of the peptide vial and let the water run slowly down the glass, not straight onto the powder.
4. Swirl the vial gently until the powder is gone. Do not shake it.
5. Write the date and 10 mg/mL on the vial, then put it in the fridge.

What Cardiogen is

Cardiogen is a synthetic tetrapeptide. The research group that made it gives its sequence as Ala-Glu-Asp-Arg (AEDR) and labels it a cardioprotector. [6]

Other names: AEDR, Ala-Glu-Asp-Arg.

How it has been given to people: no human use on record.

What to know before reading a dose

• There is no human data at all and no human dose on record. The published work is in rat tissue in culture, cultured human cells and one rat tumour study.
• No study has measured a heart outcome in a living animal or a person. The heart claim rests on rat heart tissue grown in culture [1], [2].
• All of the work comes from researchers linked to the St. Petersburg Institute of Bioregulation and Gerontology. No independent group has repeated it.
• The name is shared with an unrelated approved product. CardioGen-82 is a rubidium Rb-82 generator used in heart imaging. Search results for that product in FDA and trial databases do not refer to this peptide.
• Not an approved medicine in the US or Canada as of the check date. Health Canada warns against all unauthorized injectable peptide drugs [10].

What the evidence shows

Each finding is tagged by the kind of study it comes from. Human trials are listed first.

Side effects and warnings

Reported in human studies or on a label:

• No human side-effect report for Cardiogen was found. Health Canada lists these risks for unauthorized injectable peptide drugs in general: hormonal imbalance, mood swings, blood sugar imbalance, liver or kidney damage, blood clots and growth of cancerous tumours. [10]

Cardiogen has no approved label in the US or Canada for this use, so there is no official list of who should not use it, no interaction list and no missed-dose rule.

Storage

Keep an unmixed vial the way its label says. Once it is mixed, keep it in the fridge, do not freeze it, and write the mixing date on the vial.

Cardiogen dosage questions

Sources

Each one was opened on 10 Oct 2026 and matched to the line it supports.

1. Chalisova NI et al. [The effect of the amino acids and cardiogen on the development of myocard tissue culture from young and old rats]. Adv Gerontol 2009. PubMed 20210190.
2. Zakutskii AN et al. [The tissue-specific effect of synthetic peptides-biologic regulators in organotypic tissues culture in young and old rats]. Adv Gerontol 2006. PubMed 17152728.
3. Levdik NV et al. Tumor-modifying effect of cardiogen peptide on M-1 sarcoma in senescent rats. Bull Exp Biol Med 2009. PubMed 20396706.
4. Kheifets OV et al. [Peptidergic regulation of the expression of signal factors of fibroblast differentiation in the human prostate gland in cell aging]. Adv Gerontol 2010. PubMed 20586252.
5. Fedoreyeva LI et al. Interaction of short peptides with FITC-labeled wheat histones and their complexes with deoxyribooligonucleotides. Biochemistry (Mosc) 2013. PubMed 23581987.
6. Khavinson V et al. Transport of Biologically Active Ultrashort Peptides Using POT and LAT Carriers. Int J Mol Sci 2022. PubMed 35887081.
7. Khavinson V et al. Senescence-Associated Secretory Phenotype of Cardiovascular System Cells and Inflammaging: Perspectives of Peptide Regulation. Cells 2022. PubMed 36611900.
8. US Food and Drug Administration openFDA search of Drugs@FDA: cardiogen 2026. api.fda.gov.
9. US Food and Drug Administration Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks 2026. fda.gov.
10. Health Canada Think twice before injecting peptides bought online: unauthorized products can seriously harm you 2026. recalls-rappels.canada.ca.
11. Health Canada Health Canada Drug Product Database, active ingredient search: cardiogen 2026. health-products.canada.ca.
12. US National Library of Medicine ClinicalTrials.gov search, intervention: cardiogen 2026. clinicaltrials.gov.

Looked for and not found

• No published human study of Cardiogen of any kind. PubMed lists four records for the peptide under that name: three in cultured tissue or cells and one in rats with a transplanted tumour.
• No study in people or in live animals that measured heart function, blood pressure or any other cardiac outcome. The heart findings are from pieces of rat heart tissue in culture.
• No injected dose in any abstract read. The rat tumour study [3] says the effect was dose-dependent but its abstract states no dose.
• No PubMed abstract pairs the name Cardiogen with the sequence Ala-Glu-Asp-Arg. The pairing comes from a table in the full text of [6].
• No human half-life or other pharmacokinetic figure.
• No trial of the peptide on ClinicalTrials.gov [12].
• No approved label in the US or Canadian sources searched, so no official dose, contraindications, interaction list or storage instructions.
• No human side-effect or safety data.

Change log

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