HomePeptide dosagesDSIP (delta sleep-inducing peptide)
DSIP (delta sleep-inducing peptide) dosage: chart, reported range and vial math
The range reported for DSIP is 100 to 300 mcg, daily or a few times a week. Almost every human study gave it into a vein, dosed by body weight, and the later double-blind trials found little benefit for sleep. It is not approved in the US or Canada.
Legal status
US Not approved
Canada Not authorized
Checked 10 Oct 2026What is DSIP (delta sleep-inducing peptide)?DSIP (delta sleep-inducing peptide) dosage chart
Reported range, not a label dosePick your vial size. Each row shows the dose in milligrams or micrograms, the volume to draw and the mark on a U-100 syringe.
| Dose | Volume | Syringe units |
|---|---|---|
| 100 mcg | 0.06 mL | 6 units |
| 200 mcg | 0.12 mL | 12 units |
| 300 mcg | 0.18 mL | 18 units |
Source Reported range, not a label dose. Some reports go up to 500 mcg, in 2 to 4 week cycles. Human trials gave it into a vein, dosed by body weight. Vial math is arithmetic.
Which syringe fits each dose
For the 5 mg vial at 1.6667 mg/mL.
| Dose | Volume | Units | 0.3 mL syringe | 0.5 mL syringe | 1 mL syringe |
|---|---|---|---|---|---|
| 100 mcg | 0.06 mL | 6 units | Yes | Yes | Yes |
| 200 mcg | 0.12 mL | 12 units | Yes | Yes | Yes |
| 300 mcg | 0.18 mL | 18 units | Yes | Yes | Yes |
How long a vial lasts
Whole doses in one vial. The row for the size picked above is marked.
| Vial | 100 mcg | 200 mcg | 300 mcg |
|---|---|---|---|
| 5 mg vial | 50 doses | 25 doses | 16 doses |
How to mix a DSIP (delta sleep-inducing peptide) vial
These steps are for the 5 mg vial picked in the chart. Change the size there and the amounts here change with it.
- Wash your hands. Wipe the stopper of the peptide vial and the water vial with an alcohol swab.
- Draw 3 mL of bacteriostatic water into a syringe.
- Push the needle through the stopper of the peptide vial and let the water run slowly down the glass, not straight onto the powder.
- Swirl the vial gently until the powder is gone. Do not shake it.
- Write the date and 1.6667 mg/mL on the vial, then put it in the fridge.
| Vial | Bacteriostatic water | Concentration |
|---|---|---|
| 5 mg vial | 3 mL | 1.6667 mg/mL |
What DSIP (delta sleep-inducing peptide) is
DSIP is a nine-amino-acid peptide, Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, isolated from the cerebral venous blood of rabbits during sleep-inducing electrical stimulation of the thalamus. Its amino acid sequence was published in 1978. [1]
Other names: delta sleep-inducing peptide, emideltide, Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu.
How it has been given to people: intravenous injection or slow infusion in almost every human study found; one study gave it into the nose; no human study of injection under the skin was found.
What to know before reading a dose
- Almost all human dosing on record was intravenous, mostly set in nanomoles per kilogram, in studies of 6 to 24 people published between 1981 and 2009. No human study of injection under the skin was found.
- The results conflict. The early studies, which share a first author, reported better sleep [2], [3], [4]; two later double-blind studies in insomnia concluded the benefit was slight or unlikely to matter [5], [6].
- In an anesthesia study DSIP raised heart rate and made anesthesia lighter, not deeper [9].
- Not an approved medicine in the US or Canada as of the check date. Health Canada lists DSIP among unauthorized injectable peptide drugs it has seized [13].
- FDA compounding status: on the FDA page current as of 2026-04-22, DSIP is not in the table of substances now in category 2. It is in a separate list of substances that were in category 2 and were then withdrawn by their nominators [12].
Doses on record for DSIP (delta sleep-inducing peptide)
Every dose below was given in a human study or printed on an approved label, and each links to its source. Doses set by body weight are left that way.
| From | Where it was used | Dose | How often | For how long | Source |
|---|---|---|---|---|---|
| Human study | First human study, double-blind crossover; slow intravenous infusion in the morning | 25 nmol/kg | single dose | one day | [2] |
| Human study | Chronic insomnia, single dose before night sleep; intravenous | 25 nmol/kg | single dose | one night | [3] |
| Human study | Chronic insomnia, double-blind crossover against placebo; intravenous | 25 nmol/kg | once a night | four nights | [5] |
| Human study | Chronic insomnia, double-blind matched-pairs parallel groups; intravenous, given in the afternoon before the night recorded | 25 nmol/kg | once a day | 3 days | [6] |
| Human study | Hormone study, randomized double-blind crossover against saline; intravenous | 25 nmol/kg | single dose | one day | [7] |
| Human study | Hormone study; intravenous infusion running from 30 minutes before to 90 minutes after a CRH injection | 3 mg total | single infusion | about 2 hours | [8] |
| Human study | Hormone study; intravenous infusion, around a CRH injection in one experiment and around a meal in another | 4 mg total | single infusion | about 2 hours in the CRH experiment | [8] |
| Human study | Add-on to isoflurane anesthesia, randomized against saline; intravenous bolus, given once awake and once after induction | 25, 50 or 100 nmol/kg | two doses, one awake and one after induction of anesthesia | one day | [9] |
| Human study | Diabetic retinopathy, a product called deltalicin; given into the nose (intranasal), not injected | 0.0003 g (0.3 mg) | daily dose | two months | [10] |
What the evidence shows
Each finding is tagged by the kind of study it comes from. Human trials are listed first.
Randomized human trial
Double-blind study in 16 chronic insomniacs: slightly better sleep efficiency and shorter time to fall asleep, no change in how patients rated their sleep. The authors concluded that a major benefit was unlikely. [6]
Randomized human trial
11 healthy men, randomized crossover: plasma ACTH fell for at least 3 hours after an intravenous dose, while cortisol did not change. [7]
Randomized human trial
Given with isoflurane anesthesia to 12 women, DSIP did not deepen anesthesia. The authors concluded it made it lighter. [9]
Small human study
First human study (6 healthy volunteers): in about two hours after a morning infusion, total sleep time was 59% higher than after placebo. [2]
Small human study
6 chronic insomniacs, one intravenous dose: longer sleep with fewer interruptions. [3]
Small human study
14 chronic insomniacs, placebo-controlled and double-blind over 7 nights: night sleep and daytime alertness improved. The abstract gives no dose. [4]
Small human study
Double-blind crossover in insomniac patients over four nights: sleep time rose, but the same differences from placebo were already there at baseline, and the authors judged the benefit to be of little clinical significance. [5]
Small human study
A later study in healthy men found no effect of DSIP infusion on ACTH or cortisol after CRH or after a meal. [8]
Small human study
Diabetic retinopathy, two months of DSIP into the nose: visual evoked potential measures improved. Small study without a described placebo group. [10]
Lab study
In human and rat blood in the laboratory DSIP broke down, and the authors put the rapid disappearance of injected DSIP from blood down to that breakdown. [11]
Side effects and warnings
Reported in human studies or on a label:
- The first human study reported that a single slow intravenous infusion was well tolerated, with no side effects seen in 6 volunteers. [2]
- In 6 insomniacs there was no daytime sedation; a slight arousing effect was noted in the first hour after injection. [3]
- During isoflurane anesthesia, DSIP significantly increased heart rate and reduced heart rate variability. [9]
- FDA says it has not identified safety information for emideltide and names immune reactions and peptide impurities as possible risks of compounded product. [12]
DSIP (delta sleep-inducing peptide) has no approved label in the US or Canada for this use, so there is no official list of who should not use it, no interaction list and no missed-dose rule.
Storage
Keep an unmixed vial the way its label says. Once it is mixed, keep it in the fridge, do not freeze it, and write the mixing date on the vial.
DSIP (delta sleep-inducing peptide) dosage questions
How many units is 200 mcg of DSIP (delta sleep-inducing peptide) from a 5 mg vial?
Mixed with 3 mL of bacteriostatic water, a 5 mg vial is 1.6667 mg/mL. 200 mcg is 0.12 mL, which is 12 units on a U-100 syringe.
How much water goes in a 5 mg DSIP (delta sleep-inducing peptide) vial?
3 mL gives 1.6667 mg/mL. At that strength the doses in the chart sit at 6, 12, 18 units on a U-100 syringe.
How many doses are in a 5 mg vial?
Whole doses in one vial: 50 at 100 mcg, 25 at 200 mcg, 16 at 300 mcg.
Is DSIP (delta sleep-inducing peptide) approved in the US?
Not FDA approved. FDA lists emideltide (DSIP) among bulk substances that were in compounding category 2 and whose nominations were then withdrawn.
Is DSIP (delta sleep-inducing peptide) authorized in Canada?
No DSIP or emideltide product in the Health Canada Drug Product Database. Health Canada names DSIP among unauthorized injectable peptide drugs it has seized.
What human dosing data is there for DSIP (delta sleep-inducing peptide)?
Almost all human dosing on record was intravenous, mostly set in nanomoles per kilogram, in studies of 6 to 24 people published between 1981 and 2009. No human study of injection under the skin was found.
Sources
Each one was opened on 10 Oct 2026 and matched to the line it supports.
- Schoenenberger GA et al. The delta EEG (sleep)-inducing peptide (DSIP). XI. Amino-acid analysis, sequence, synthesis and activity of the nonapeptide. Pflugers Arch 1978. PubMed 568769.
- Schneider-Helmert D et al. Acute and delayed effects of DSIP (delta sleep-inducing peptide) on human sleep behavior. Int J Clin Pharmacol Ther Toxicol 1981. PubMed 6895513.
- Schneider-Helmert D et al. The influence of synthetic DSIP (delta-sleep-inducing-peptide) on disturbed human sleep. Experientia 1981. PubMed 7028502.
- Schneider-Helmert D Effects of delta-sleep-inducing peptide on 24-hour sleep-wake behaviour in severe chronic insomnia. Eur Neurol 1987. PubMed 3622582.
- Monti JM et al. Study of delta sleep-inducing peptide efficacy in improving sleep on short-term administration to chronic insomniacs. Int J Clin Pharmacol Res 1987. PubMed 3583493.
- Bes F et al. Effects of delta sleep-inducing peptide on sleep of chronic insomniac patients. A double-blind study. Neuropsychobiology 1992. PubMed 1299794.
- Bjartell A et al. Reduction of immunoreactive ACTH in plasma following intravenous injection of delta sleep-inducing peptide in man. Psychoneuroendocrinology 1989. PubMed 2554357.
- Spath-Schwalbe E et al. Delta-sleep-inducing peptide does not affect CRH and meal-induced ACTH and cortisol secretion. Psychoneuroendocrinology 1995. PubMed 7777652.
- Pomfrett CJ et al. Delta sleep-inducing peptide alters bispectral index, the electroencephalogram and heart rate variability when used as an adjunct to isoflurane anaesthesia. Eur J Anaesthesiol 2009. PubMed 19142086.
- Kresiun NV et al. [Using deltalicin for the treatment of patients with diabetic retinopathy]. Eksp Klin Farmakol 2014. PubMed 25739189.
- Graf MV et al. Degradation and aggregation of delta sleep-inducing peptide (DSIP) and two analogs in plasma and serum. Peptides 1987. PubMed 3628078.
- US Food and Drug Administration Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks 2026. fda.gov.
- Health Canada Think twice before injecting peptides bought online: unauthorized products can seriously harm you 2026. recalls-rappels.canada.ca.
- Health Canada Health Canada Drug Product Database, active ingredient search: emideltide 2026. health-products.canada.ca.
- World Anti-Doping Agency The Prohibited List 2026. wada-ama.org.
Looked for and not found
- No human study of DSIP injected under the skin or into muscle. A PubMed search for those routes in people returned one intranasal letter with no abstract and one mouse study.
- No human half-life figure. One laboratory study attributes the rapid disappearance of injected DSIP from blood to breakdown but gives no number in its abstract [11], so half_life is left out.
- The 7-night insomnia study [4] gives no dose in its abstract.
- No DSIP trial registered on ClinicalTrials.gov: searches for DSIP, emideltide and delta sleep-inducing peptide as the intervention returned no trial of the peptide.
- No approved label, so no official dose, contraindications, interaction list or storage instructions.
- Not named on the WADA Prohibited List (page checked 2026-10-10, [15]). The list also has an open class, S0, for substances with no current approval by any governmental health authority for human therapeutic use; whether it applies to this compound was not determined here.
Change log
10 Oct 2026Page published. 15 sources checked. Vial math added for the 5 mg vial.
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