Testagen dosage: chart, reported range and vial math

How to mix a Testagen vial

These steps are for the 20 mg vial picked in the chart. Change the size there and the amounts here change with it.

1. Wash your hands. Wipe the stopper of the peptide vial and the water vial with an alcohol swab.
2. Draw 2 mL of bacteriostatic water into a syringe.
3. Push the needle through the stopper of the peptide vial and let the water run slowly down the glass, not straight onto the powder.
4. Swirl the vial gently until the powder is gone. Do not shake it.
5. Write the date and 10 mg/mL on the vial, then put it in the fridge.

What Testagen is

Testagen is a synthetic tetrapeptide, Lys-Glu-Asp-Gly (KEDG). The researchers who made it say it was built from the amino acid composition of peptide extracts of the pituitary gland. [1]

Other names: KEDG, Lys-Glu-Asp-Gly.

How it has been given to people: no human use on record.

What to know before reading a dose

• There is no human data at all and no human dose on record. The published work is in chickens and hens and in cells in culture.
• No published study shows that Testagen raises testosterone. The name and the label of male reproductive regulator come from the group that made it [6], with no study cited.
• The animal work comes from one circle of researchers linked to the St. Petersburg Institute of Bioregulation and Gerontology. No independent group has repeated it.
• The name is shared with an unrelated product. Testagen TDS is a testosterone skin spray that appears in a US drug label record and in a ClinicalTrials.gov entry [11]. Neither refers to this peptide.
• Not an approved medicine in the US or Canada as of the check date. Health Canada warns against all unauthorized injectable peptide drugs [9].

What the evidence shows

Each finding is tagged by the kind of study it comes from. Human trials are listed first.

Side effects and warnings

Reported in human studies or on a label:

• No human side-effect report for Testagen was found. Health Canada lists these risks for unauthorized injectable peptide drugs in general: hormonal imbalance, mood swings, blood sugar imbalance, liver or kidney damage, blood clots and growth of cancerous tumours. [9]

Testagen has no approved label in the US or Canada for this use, so there is no official list of who should not use it, no interaction list and no missed-dose rule.

Storage

Keep an unmixed vial the way its label says. Once it is mixed, keep it in the fridge, do not freeze it, and write the mixing date on the vial.

Testagen dosage questions

Sources

Each one was opened on 10 Oct 2026 and matched to the line it supports.

1. Fedoreyeva LI et al. Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Mosc) 2011. PubMed 22117547.
2. Kuznik BI et al. Effect of tetrapeptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on the structure and function of the thyroid gland in neonatally hypophysectomized chickens. Bull Exp Biol Med 2008. PubMed 19024016.
3. Kuznik BI et al. Effects of peptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on hormonal activity and structure of the thyroid gland in hypophysectomized young chickens and old hens. Bull Exp Biol Med 2011. PubMed 22268052.
4. Pateyk AV et al. Effect of peptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on the morphology of the thymus in hypophysectomized young and old birds. Bull Exp Biol Med 2013. PubMed 23658898.
5. Dobritescu A et al. The Inhibitory Effect and Adsorption Properties of Testagen Peptide on Copper Surfaces in Saline Environments: An Experimental and Computational Study. Molecules 2025. PubMed 40807317.
6. Khavinson V et al. Transport of Biologically Active Ultrashort Peptides Using POT and LAT Carriers. Int J Mol Sci 2022. PubMed 35887081.
7. US Food and Drug Administration openFDA search of Drugs@FDA: testagen 2026. api.fda.gov.
8. US Food and Drug Administration Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks 2026. fda.gov.
9. Health Canada Think twice before injecting peptides bought online: unauthorized products can seriously harm you 2026. recalls-rappels.canada.ca.
10. Health Canada Health Canada Drug Product Database, active ingredient search: testagen 2026. health-products.canada.ca.
11. ClinicalTrials.gov Product Transference Study of Testagen TDS-Testosterone 2026. clinicaltrials.gov. Trial NCT02733133.

Looked for and not found

• No published human study of Testagen of any kind. PubMed lists two records under the name (a cell and DNA binding study and a copper corrosion paper) and a handful under the sequence Lys-Glu-Asp-Gly, all in birds.
• No study measuring testosterone, luteinizing hormone, sperm or any other male reproductive outcome, in people or in animals. The bird studies measured thyroid hormones and thymus structure.
• No dose in any abstract read, human or animal.
• No human half-life or other pharmacokinetic figure.
• No trial of the peptide on ClinicalTrials.gov. The one result for testagen is a testosterone skin spray with the same name [11].
• No approved label in the US or Canadian sources searched, so no official dose, contraindications, interaction list or storage instructions.
• No human side-effect or safety data.

Change log

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