HomePeptide dosagesSemaglutide5 mg vial
Semaglutide 5 mg vial: mixing and syringe units
A 5 mg vial of Semaglutide mixed with 2 mL of bacteriostatic water is 2.5 mg/mL. At that strength 0.25 mg is 10 units on a U-100 syringe and 2.4 mg is 96 units. The doses on this page are the schedule printed on the approved label.
5 mg vial
Water 2 mL
Concentration 2.5 mg/mL
US Approved, prescription
Canada Authorized, prescription
Checked 10 Oct 2026Semaglutide 5 mg draw chart
Each row is one dose, the volume to draw and where that sits on a U-100 insulin syringe. The last three columns show which syringe sizes can hold it in one draw.
| Dose | Volume | Syringe units | 0.3 mL syringe | 0.5 mL syringe | 1 mL syringe |
|---|---|---|---|---|---|
| 0.25 mg Weeks 1 to 4 | 0.10 mL | 10 units | Yes | Yes | Yes |
| 0.5 mg Weeks 5 to 8 | 0.20 mL | 20 units | Yes | Yes | Yes |
| 1 mg Weeks 9 to 12 | 0.40 mL | 40 units | No | Yes | Yes |
| 1.7 mg Weeks 13 to 16 | 0.68 mL | 68 units | No | No | Yes |
| 2.4 mg Week 17 on | 0.96 mL | 96 units | No | No | Yes |
Source Schedule from the FDA label. The US label also allows up to 7.2 mg a week after 4 weeks on 2.4 mg. Vial math is arithmetic.
How to mix a 5 mg Semaglutide vial
- Wash your hands. Wipe the stopper of the Semaglutide vial and the water vial with an alcohol swab.
- Draw 2 mL of bacteriostatic water into a syringe.
- Push the needle through the stopper of the peptide vial and let the water run slowly down the glass, not straight onto the powder.
- Swirl the vial gently until the powder is gone. Do not shake it.
- Write the date and 2.5 mg/mL on the vial, then put it in the fridge.
If you add a different amount of water
More water makes each dose a bigger, easier draw. Less water makes it smaller. The milligrams in the vial do not change.
| Water added | Concentration | 0.25 mg | 0.5 mg | 1 mg | 1.7 mg | 2.4 mg |
|---|---|---|---|---|---|---|
| 1 mL | 5 mg/mL | 5 units | 10 units | 20 units | 34 units | 48 units |
| 2 mL | 2.5 mg/mL | 10 units | 20 units | 40 units | 68 units | 96 units |
| 3 mL | 1.6667 mg/mL | 15 units | 30 units | 60 units | 102 units | 144 units |
The marked row is the amount used in the chart above. Red cells are draws under 5 units, which are hard to read, or over 100 units, which one 1 mL syringe cannot hold.
How many doses are in the vial
| Dose | Whole doses in a 5 mg vial | At once weekly |
|---|---|---|
| 0.25 mg | 20 doses | 20 weeks |
| 0.5 mg | 10 doses | 10 weeks |
| 1 mg | 5 doses | 5 weeks |
| 1.7 mg | 2 doses | 2 weeks |
| 2.4 mg | 2 doses | 2 weeks |
Semaglutide 5 mg vial questions
How many units is 0.25 mg of Semaglutide from a 5 mg vial?
With 2 mL of bacteriostatic water the vial is 2.5 mg/mL. 0.25 mg is 0.10 mL, which is 10 units on a U-100 syringe.
How many units is 0.5 mg of Semaglutide from a 5 mg vial?
With 2 mL of bacteriostatic water the vial is 2.5 mg/mL. 0.5 mg is 0.20 mL, which is 20 units on a U-100 syringe.
How many units is 1 mg of Semaglutide from a 5 mg vial?
With 2 mL of bacteriostatic water the vial is 2.5 mg/mL. 1 mg is 0.40 mL, which is 40 units on a U-100 syringe.
How much bacteriostatic water goes in a 5 mg Semaglutide vial?
2 mL is the amount used on this page. It gives 2.5 mg/mL and puts the doses at 10, 20, 40, 68, 96 units. Any amount works as long as the math is redone for it.
What if I added 1 mL instead of 2 mL?
The vial would be 5 mg/mL instead of 2.5 mg/mL, so 0.25 mg would be 5 units instead of 10. The dose in the syringe is the same; only the volume changes.
How many doses are in a 5 mg Semaglutide vial?
Whole doses in one vial: 20 at 0.25 mg, 10 at 0.5 mg, 5 at 1 mg, 2 at 1.7 mg, 2 at 2.4 mg.
More on Semaglutide
This page is only the math for one vial size. Where the doses come from, what the studies found, the side effects and the sources are on the main page.
Semaglutide dosageSemaglutide 2 mg vialWhat Semaglutide isHow to mix a vialHow to read a syringe
DosageFacts is an information site. It is not medical advice. Talk to a licensed professional before using any medication.